Methods and equipment
Contact
Director of the Department of Ophthalmology
Head of the Laboratory for Experimental Ophthalmology
Office at Pius-Hospital Oldenburg
Address
Methods and equipment
The focus of our research includes methods of cell culture, molecular biology and histology. Below you will find a list of our currently available equipment.
Cell culture
Devicee | Techniques / Methods |
Heracell Vios 250i CO2 incubator | Cultivation of primarycells (sensitive neurones, lacrimal gland cells) and cell lines |
ico50med Memmert O2 incubator | Cultivation of mesenchymal stem cells |
Thermo Scientific Safe 2020 safety cabinet | |
VWR MegaStar 1.6 centrifuge | |
Dismembrator for tissue disruption: Star Beater oscillating mill | Preparation/Tissue disruption of samples such as corneas and lacrimal glands |
JULI with GFP fluorescence | Live-cell Microscopy of cell culture samples |
Zeiss Axio Vert A1 | Inverted fluorescence microscope with camera |
Bioreactor | Cultivation of 3D constructs under in vivo-like conditions |
Nitrogen storage tank MVE 510 AF-GB BB MDD | Storage of cells and tissue samples under standardised conditions in the liquid phase of nitrogen |
Molecular biology
Devices | Techniques/Methods |
Quant Studio 3 | Quantitative real-time PCR |
Biometra TAdvanced Twin 48G | Gradient PCR, PCR |
Nano Drop One C | Photometer |
Horizontal gel system Mini and Midi | RNA and DNA gels |
Plate centrifuge | 96well plates |
Heraeus Fresco 21 | Refrigerated centrifuge |
PCMT 24 x 2 ml | Heating/shaking block |
IKA CMAG HS 7 Control | Heating/magnetic stirrer |
Stuart See-Saw Rocker SSL4 | Rocking table small |
Edmund-Bühler WS10 | Rocking table large |
Rotator Genie magnetic platform | Orbital shaker |
Mini table centrifuges Sprout | |
Vortex Genie 2 | Vortexer |
Protein biochemistry/histology
Devices | Techniques/Methods |
Chemidoc MP BioRad | Western blot, gel electrophoresis |
BioRad Trans-Blot Semi-Dry Transfer Cell and BioRad Power Pac Basic and high voltage | Westernblot, SDS-Page, Tank-Blot, Semi-Dry-Blot |
Leica Multicut incl. CoolClamp | Microtome for paraffin tissue sections |
Leica HistoCore Arcadia H | Paraffin casting station |
Leica TP 1020 | Automatic tissue dehydrator |
Paraffin stretching bath | |
Heating plate for slides | Drying of the paraffin sections |
Binder ED115 drying oven | Drying the paraffin sections |
Satorius Entris 224-1S | Analysing balance |
Mettler-Toledo seven compact S210 bio | pH meter |
Standard microscope, VisiScope®, BL214 | Microscope for monitoring DAB staining |